Kovid testing 19
Testing for kovid 19 or Test for virus detection in coronavirus disease 2019 (acronym kovid 19) or diagnostic test for detection of SARS-CoV-2 virus is, as its name suggests, a diagnostic tool used in case of suspicion that a person is infected respiratory virus SARS-CoV-2, the causative agent of coronavirus disease (Kovid-19), which is spreading globally after the new virus was first identified in Wuhan, a city in China, in late 2019. In principle, the test results are given within 12-24 hours of sampling.
Public health experts have pointed to the fact that the rapid identification and isolation of infected people is a crucial factor in limiting the spread of the virus, which can be deadly for the elderly or those with other health problems. The global mortality rate from kovida 19, according to the World Health Organization, was estimated in March 2020 at around 3.4%. Because many milder cases of the disease will remain undiagnosed, the actual mortality rate may be much lower. was positive, as well as when someone who became infected was recognized by a test before manifesting symptoms? Pinsky and his colleagues for making eng. The Stanford Medicine COVID-19 test currently used in the United States used primers and probes developed in a laboratory in Germany that has studied the SARS and MERS coronaviruses for many years. They then optimized the use of these diagnostic tools to meet U.S. testing standards. According to researchers from Stanford:
If the test is positive - it means that the tested person is infected with the corona virus. But this does not necessarily mean that SARS-CoV-2 is the cause (or only cause) of the disease. Co-infection with another virus or bacteria can cause identical symptoms.
If the test is negative - other factors are important to confirm that the test person has not contracted kovid 19, such as medical history and clinical examination, because the test person may actually be infected with the virus, but his presence in the sample was not detected.
Molecular tests for the detection of SARS-CoV-2 virus as a cause of kovid 19
Currently, there are several tests for the detection of SARS-CoV-2 virus in the sales and application phase, as well as in the research phase. Some tests can only detect a new virus, and some can detect other strains (e.g., SARS-CoV) that are genetically similar.
The test uses a technique called reverse transcriptase polymerase chain reaction (acronym RT-PCR) to quickly detect the presence of viral RNA in nasal swabs of potentially infected individuals. It is used when the target sequence is viral RNA that must first be transcribed into complementary DNA using a reverse transcriptase enzyme. RT-PCR is applied for
detection of numerous RNA viruses, including SARS-CoV-2, the causative agent of kovid 19.
RT-PCR uses short DNA prostheses called primers that bind tightly and specifically only to the corresponding sequences in SARS-CoV-2 RNA. An enzyme called reverse transcriptase then converts viral RNA into complementary DNA, and as the reaction continues, an enzyme called polymerase is used to generate billions of copies of DNA that can be detected by fluorescently labeled molecules called probes. RNA that encodes a protein called envelope protein, which is located in the membrane surrounding the virus and plays an important role in the life cycle of the virus, including shoots from infected host cells. It then confirms the positive result by testing a gene encoding a second protein called RNA-dependent RNA polymerase. The test on CDC screens is used to determine the third gene encoding the protein that forms the virus.